Several potential marker candidates for Parkinson’s disease (PD) in cerebrospinal fluid (CSF) have been identified. These include α-synuclein, a major constituent of the intracellular aggregates Lewy bodies, a neuropathological hallmark of PD, and others. The extracellular presence of α-synuclein in blood, CSF, saliva, and conditioned media motivated the hypothesis that the quantification of CSF α-synuclein is a biomarker for α-synuclein-related disorders. We here describe the development of an electrochemiluminescence-based assay by conversion of an established ELISA for quantification of α-synuclein. The assay not only works with CSF but can also be used to quantify α-synuclein in different biological fluids, i.e., whole blood and blood products, saliva, as well as brain homogenates and cell culture material.
CITATION STYLE
Kruse, N., & Mollenhauer, B. (2019). Quantification of alpha-synuclein in biological fluids by electrochemiluminescence-based detection. In Methods in Molecular Biology (Vol. 1948, pp. 59–68). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9124-2_6
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