Pepper is a recalcitrant plant for Agrobacterium -mediated genetic transformation. Several obstacles to genetic transformation remain such as extremely low transformation rates; the choice of correct genotype is critical; and there is a high frequency of false positives due to direct shoot formation. Here, we report a useful protocol with a suitable selection method. The most important aspect of the pepper transformation protocol is selecting shoots growing from the callus, which is referred to as callus-mediated shoot formation. This protocol is a reproducible and reliable system for pepper transformation.
CITATION STYLE
Min, J., Hee Shin, S., Mi Jeon, E., Mi Park, J., Young Hyun, J., & Hark Harn, C. (2015). Pepper, Chili (Capsicum annuum). Methods in Molecular Biology, 1223, 311–320. https://doi.org/10.1007/978-1-4939-1695-5_25
Mendeley helps you to discover research relevant for your work.