Bacterial cold shock proteins (CSPs) function as RNA chaperones that destabilize RNA secondary s tructures. A quadruple mutant of Escherichia coli (cspA, cspB, cspG, and cspE) displays a cold-sensitive growth phenotype. Plant cold shock domain (CSD) proteins have been shown to complement the coldsensitive phenotype of the E. coli mutant and to share a function with E. coli CSPs as RNA chaperones. This methodology, which is detailed here, can be utilized to reveal or probe the RNA chaperone activity of heterologous proteins.
CITATION STYLE
Kim, M. H., & Imai, R. (2015). Determination of RNA chaperone activity using an Escherichia coli mutant. Methods in Molecular Biology, 1259, 117–123. https://doi.org/10.1007/978-1-4939-2214-7_8
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