Study on fermentation conditions of palm juice vinegar by Response Surface Methodology and development of a kinetic model

Abstract

Natural vinegar is one of the fermented products which has some potentiality with respect to a nutraceutical standpoint. The present study is an optimization of the fermentation conditions for palm juice vinegar production from palm juice (Borassus flabellifer) wine, this biochemical process being aided by Acetobacter aceti (NCIM 2251). The physical parameters of the fermentation conditions such as temperature, pH, and time were investigated by Response Surface Methodology (RSM) with 2 3 factorial central composite designs (CCD). The optimum pH, temperature and time were 5.5, 30°C and 72 hrs for the highest yield of acetic acid (68.12 g/L). The quadratic model equation had a R 2 value of 0.992. RSM played an important role in elucidating the basic mechanisms in a complex situation, thus providing better process control by maximizing acetic acid production with the respective physical parameters. At the optimized conditions of temperature, pH and time and with the help of mathematical kinetic equations, the Monod specific growth rate (μ max= 0.021 h -1), maximum Logistic specific growth rate (μ' max = 0.027 h -1) and various other kinetic parameters were calculated, which helped in validation of the experimental data. Therefore, the established kinetic models may be applied for the production of natural vinegar by fermentation of low cost palm juice.