Identification of MRSA(methicillin resistant Staphylococcus aureus) by mecA gene

Abstract

Staphylococcus aureus is a bacterium that may be found in the skin and upper respiratory system. Although S.aureus is a normal flora, it has evolved into an opportunistic pathogen that causes numerous infections that are difficult to cure due to the presence of virulence genes. The goal of this work is to identify MRSA in bacterial isolates using the mecA gene. In the current investigation, 213 samples were gathered from various clinical sources from patients hospitalized to various hospitals in Baghdad. The isolates were identified as S.aureus using normal bacteriological methods and verified using a molecular approach based on the nuc gene. Due to the many species of staphylococci that were expressed for varying resistance levels, the conventional approach was the best way for finding Methicillin resistant gene in S. aureus (mecA). Finally, mecAgene was found to be present in 76.67 % of bacterial isolates.