Identification of Posttranslational Modification by Mass Spectrometry

Abstract

Many chemically distinct types of posttranslational modification of proteins are known. These include the wide variety of prosthetic groups at enzymes' active sites, acylations at the N- and the C-terminus and at internal sites in proteins. In this chapter, the examples of mass-spectrometry techniques employed for analysis of glycosylation and disulphide bonds are given. Techniques for identification of sites of phosphorylation are in Chapter 44.