Embryoid and Callus Formation from Pollen Grains of Eggplant and Pepper by Anther Culture.

Abstract

Effective methods for num melongena L.) and the production pepper (Capsicum of haploid annuum plants by culturing L.) were studied. anthers Anthers grains trations at the uninucleate of 2,4-D and kinetin stage and were then plated exposed on MS medium supplied with to high and low temperatures. of eggplant containing (Sola- pollen various Embryoids concen- from pollen grain and adventitious shoots from calli were transferring to a MS medium. Results obtained for eggplant were as follows: obtained, and they grew 1. Anthers tin and treated cultured with on MS medium supplied 35•Ž for 24 hr formed calli with and 0.1 mg•Eliter-1 embryoids at of both the highest low 2. temperature Regeneration treatment (5•Ž for 24 or 48 hr) was ineffective of embryoid and adventitious buds from calli or rather was induced to plantlets upon 2,4-D and frequency, kine- but inhibitory. by transferring calli 0.05 to MS mg•Eliter-1 medium NAA. without phytohormones or with 1 mg•Eliter-1 kinetin plus 0 or 3. Plantlets vigorously. Results for on MS media pepper were supplied with as follows: 0.8% agar and 5~8% sucrose grew normally and 4. Calli were formed at high frequency on MS media supplied with 0.2 mg•Eliter-1 2,4-D plus 0.1 mg•Eliter-1 kinetin embryoids were directly 0.02 mg•Eliter-1 kinetin or or 0.1 mg•Eliter-1 both of 2,4-D formed at high frequency 0.004 mg•Eliter-1 2,4-D plus 0.1 and kinetin. On the other on MS media supplied mg•Eliter-1 kinetin. hand, with Introduction