Quantitative profiling of chaperone/client interactions with LUMIER assay

Abstract

Chaperones associate with hundreds or thousands of diverse client proteins and regulate their function. Chaperone/client interactions are generally very transient and involve a highly orchestrated assembly and disassembly of regulatory co-factors. This poses specific challenges for identifying and characterizing these interactions in a scalable and sensitive manner. LUMIER assay, which takes advantage of the high sensitivity and linear range of luminescence-based detection, has proven to be an ideal assay to quantitatively profile chaperone/client interactions in a high-throughput manner. This article provides step-by-step instructions for quantitatively profiling these interactions with LUMIER.