The action of a water-soluble carbodiimide on adenosine-5'-polyphosphates

Abstract

When aqueous solutions of adenosine-5′-aono-, di-, or triphosphates are treated with a water soluble carbodiimide the major product is the expected diadenosine-5′-5′-polyphosphate. The yields of these pyrophosphates are greatly increased in the presence of the Mg2+ ion. Adenosine-5′-tetraphosphate behaves differently. The major product is adenosine-5′-monophosphate. We believe that this hydrolysis occurs via a cyclic trimetaphosphate intermediate. © 1987 IRL Press Limited, Oxford, England.