Ca 2+ -dependent functions in Peptidoglycan-stimulated mouse dendritic Cells

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Abstract

Peptidoglycans (PGN) from bacterial cell walls may modify the course of an infection with bacterial pathogens. The present study explored the effect of PGN on cytosolic Ca 2+ activity, cytokine production and phagocytosis of mouse dendritic cells (DCs), essential cells in the initiation and direction of antigen-specific T cell responses. Exposure of DCs to PGN was followed by a rapid increase in cytosolic Ca 2+ activity ([Ca 2+ ] i ), which was due to Ca 2+ release from intracellular stores and influx of extracellular Ca 2+ across the cell membrane. In DCs isolated from Toll-like receptor 2 (TLR2) deficient mice the effect of PGN on [Ca 2+ ] i was dramatically impaired. The PGN-induced increase of [Ca 2+ ] i was dependent on voltage-gated K + (Kv) channel activity. PGN-induced increase of [Ca 2+ ] i was significantly blunted by margatoxin (MgTx) and perhexiline maleate (PM), inhibitors of Kv1.3 and Kv1.5, respectively. PGN further stimulated the release of tumour necrosis factor α (TNFα), interleukin-12 (IL-12) and interleukin-10 (IL-10), an effect significantly blunted by PM and the specific blocker of store-operated Ca 2+ channels SKF-96365. Moreover, phagocytic capacity was dramatically increased in PGN-stimulated DCs in the presence of either Kv channel inhibitors or SKF-96365. The observations disclose Ca 2+ and Kv channel-dependent cytokine production and phagocytosis in PGN-stimulated DCs. Copyright © 2009 S. Karger AG, Basel.

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Xuan, N. T., Shumilina, E., Matzner, N., Zemtsova, I. M., Biedermann, T., Goetz, F., & Lang, F. (2009). Ca 2+ -dependent functions in Peptidoglycan-stimulated mouse dendritic Cells. Cellular Physiology and Biochemistry, 24(3–4), 167–176. https://doi.org/10.1159/000233243

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