Associations between genomic merit for daughter pregnancy rate of Holstein cows and metabolites postpartum and estrus characteristics

Abstract

Genetic selection of Holstein cattle in the past 2 decades has seen an increased attention to fertility traits. Our hypotheses were that genomic merit for daughter pregnancy rate (GDPR) is positively associated with metabolic responses, hazard of estrus, and estrus characteristics. Pregnant heifers (n = 821) from one herd that were genotyped within 2 mo of birth (Clarifide, Zoetis, Parsippany, NJ) were fitted with automated monitoring devices (SCR Inc., Netanya, Israel) −21 ± 14 d relative to calving. Estrus characteristics recorded from calving to 62 d postpartum were evaluated. Blood samples were collected weekly from a subsample (n = 499) of cows, from 7 to 28 d postpartum, for determination of insulin-like growth factor-1, glucose, and nonesterified fatty acids. Cows received artificial insemination or embryo transfer following detected estrus and those not detected in estrus were submitted to an ovulation synchronization protocol starting at 75 d in milk. Linear and quadratic associations between GDPR and outcomes were analyzed, but when appropriate, results are presented according to GDPR quartile (Q1 = −1.8 to 0.8; Q2 = 0.9 to 1.7; Q3 = 1.8 to 2.5; Q4 = 2.6 to 5.9) based on the parameter estimates of the multivariable models. Genomic merit for daughter pregnancy rate was positively associated with insulin-like growth factor-1 (Q1 = 24.3 ± 0.2; Q2 = 26.8 ± 0.2; Q3 = 28.2 ± 0.2; Q4 = 30.6 ± 0.3 ng/mL) and glucose (Q1 = 67.0 ± 0.1; Q2 = 69.1 ± 0.2; Q3 = 69.6 ± 0.2; Q4 = 70.8 ± 0.2 mg/dL) concentrations, but GDPR was negatively associated with nonesterified fatty acid concentration (Q1 = 281.2 ± 4.9; Q2 = 262.0 ± 5.9; Q3 = 239.3 ± 5.0; Q4 = 221.6 ± 4.7 μmol/L). A positive association was observed between GDPR and hazard of estrus [adjusted hazard ratio and 95% confidence interval = 1.16 (1.06, 1.28)] and number of estrus events (Q1 = 0.50 ± 0.03; Q2 = 0.62 ± 0.04; Q3 = 0.74 ± 0.05; Q4 = 0.86 ± 0.06) within 62 d postpartum, duration of estrus (Q1 = 14.10 ± 0.04; Q2 = 14.48 ± 0.04; Q3 = 14.67 ± 0.04; Q4 = 14.98 ± 0.04 h), probability of activity peak (0 = no estrus, 100 = maximum activity) ≥86 (Q1 = 0.80 ± 0.03; Q2 = 0.83 ± 0.02; Q3 = 0.83 ± 0.03; Q4 = 0.85 ± 0.2), and probability of heat index ≥86 (Q1 = 0.77 ± 0.04; Q2 = 0.81 ± 0.05; Q3 = 0.83 ± 0.03; Q4 = 0.86 ± 0.03). Conversely, GDPR was negatively associated with rumination nadir at estrus (Q1 = −35.5 ± 0.1; Q2 = −37.0 ± 0.1; Q3 = −38.0 ± 0.1; Q4 = −39.6 ± 0.1 min). We detected a positive association between GDPR and hazard of pregnancy (adjusted hazard ratio = 1.11, 95% confidence interval = 1.03, 1.19). Selection for GDPR may improve the hormonal and metabolic status of cows postpartum, leading to earlier resumption of cyclicity, and may improve detection of estrus in commercial herds because it was positively associated with estrus characteristics.