Differential regulation of CD11b on γδ T cells and monocytes in response to unripe apple polyphenols

Abstract

Leukocyte adhesion and migration are mediated partially by CD11b/CD18 (membrane-activated complex-1, CR3). Earlier studies have demonstrated a role for green tea polyphenols in down-regulating CD11b on CD8+ T cells and monocytes. We have shown recently a stimulatory effect of unripe apple polyphenols (APP) on γδ T cells. Thus, we compared the effect of APP on bovine γδ T cell and monocyte CD11b expression. Purified bovine monocytes and monocyte-depleted PBLs were cultured with APP. CD11b levels decreased on monocytes in response to APP. In contrast, a γδ T cell subset responded to APP by up-regulating CD11b. The CD11b regulation was not seen on γδ T cells or monocytes treated with APP fractions depleted of tannins. The APP-induced down-regulation of CD11b on monocytes was inhibited by an anti-CD11b mAb, consistent with previous studies showing that polyphenols bind CD11b. As expected, the anti-CD11b mAb had no effect on the APP response in resting γδ T cells, as these cells lacked CD11b. Consistent with the changes in surface CD11b expression, APP-treated γδ T cells showed increased adherence to pastic, whereas monocyte adhesion was reduced. APP also induced cytokine gene expression in γδ T cells. Some polyphenols are thought of as anti-inflammatory agents; however, these data, as well as other ongoing studies, indicate they have a proinflammatory effect on γδ T cells. In vivo, plant polyphenols may enhance γδ T cell migration and function at sites of inflammation, where they could induce rapid, immune-regulatory and innate-like immune responses.