Tungstate activates BK channels in a β subunit-and Mg 2+-dependent manner: Relevance for arterial vasodilatation

Abstract

Aims Tungstate reduces blood pressure in experimental animal models of both hypertension and metabolic syndrome, although the underlying mechanisms are not fully understood. Given that the large-conductance voltage-and Ca 2+-dependent K + (BK) channel is a key element in the control of arterial tone, our aim was to evaluate whether BK channel modulation by tungstate can contribute to its antihypertensive effect. Methods and results Patch-clamp studies of heterologously expressed human BK channels (α β 1-4 subunits) revealed that cytosolic tungstate (1 mM) induced a significant left shift (∼20 mV) in the voltage-dependent activation curve only in BK channels containing αβ 1 or αβ 4 subunits, but reduced the amplitude of K + currents through all BK channels tested. The β 1-dependent activation of BK channels by tungstate was enhanced at cytosolic Ca 2+ levels reached during myocyte contraction, and prevented either by removal of cytosolic Mg 2+ or by mutations rendering the channel insensitive to Mg 2+. A lower concentration of tungstate (0.1 mM) induced voltage-dependent activation of the vascular BKαβ 1 channel without reducing current amplitude, and consistently exerted a vasodilatory action on wild-type but not on β1-knockout mouse arteries pre-contracted with endothelin-1. Conclusion Tungstate activates BK channels in a β subunit-and Mg 2+-dependent manner and induces vasodilatation only in mouse arteries that express the BK β 1 subunit. Published on behalf of the European Society of Cardiology. All rights reserved. © 2012 The Author.