Pharmaceutical Applications of Gas Chromatography

Abstract

Gas chromatography is an analytical tool for the separation of compounds in complex mixtures based on the polarity of compounds. Separation is achieved only for compounds that are volatile or that can be made volatile on derivatization of the compound using derivatizing agents. This is one of the widely accepted tools for the separation of compounds because of its simplicity, sensitivity, and effectiveness. The principle of separation of compounds depends on the partitioning behaviour difference between mobile and stationary phase, the sample is carried by a moving gas stream through a tube packed with a finely divided solid or may be coated with a film of a liquid. Different types of columns having a various composition of stationary phase are been used for the separation of different classes of compounds mixture or sample in a suitable solvent is introduced through the injector maintained at higher temperature which is capable of volatilizing the compound into the column.