Optochemical Control of Cell Contractility in Drosophila Embryos

Abstract

Cell shape changes based on actomyosin contractility provide a driving force in tissue morphogenesis. The temporally and spatially coordinated constrictions of many cells result in changes in tissue morphology. Given the networks of complex and mutual cellular interactions, the mechanisms underlying the emergence in tissue behavior are challenging to pinpoint. Important in the analysis of such interactions are novel methods for noninvasive interference with single-cell resolution and sub-minute timescale temporal control. Here we characterize an optochemical approach of Ca2+ uncaging to control cell contractility in Drosophila embryos. We describe in detail the method of sample preparation, microinjection, Ca2+ uncaging, and data analysis.