Studying signal transduction in hypertension model systems in vitro will include several steps: (1) develop the cell culture model and induce hypertensive changes, (2) observe kinase activation, (3) manipulate signal transduction pathways, and (4) observe physiologic outputs. This chapter will provide the reader with overviews of the techniques our lab uses to inhibit signaling pathways with inhibitory RNAs and the outputs we use to monitor the effects.
CITATION STYLE
Elliott, K. J., & Eguchi, S. (2017). In vitro analysis of hypertensive signal transduction: Kinase activation, kinase manipulation, and physiologic outputs. In Methods in Molecular Biology (Vol. 1527, pp. 201–211). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6625-7_16
Mendeley helps you to discover research relevant for your work.