Uridine addition at the 3’ end of RNAs (i.e., uridylation) emerges as a critical posttranscriptional modifi cation promoting RNA degradation. Uridylation has been notably linked to the degradation of small RNAs, correlated with the 5’ shortening of RISC-cleaved transcripts and the degradation of mRNAs. We describe here a method based on 3’ RACE (3’ Rapid Amplifi cation of cDNA End) PCR that has been successfully used to investigate nucleotide addition at the 3’ end of RISC-cleaved transcripts and full-length mRNAs in plants.
CITATION STYLE
Sement, F. M., & Gagliardi, D. (2014). Detection of uridylated mRNAs. Methods in Molecular Biology, 1125, 43–51. https://doi.org/10.1007/978-1-62703-971-0_4
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