In the present study, we examined intraosseous blood vessel parameters of the tibial metaphysis in mice using microcomputed tomography (μCT) to investigate the relationship between post-nerve-injury osteoporosis and local intraosseous blood vessel volume and number. Mice were randomly divided into groups receiving spinal cord injury (SCI), sciatic nerve resection group (NX), or intact controls (30 mice/group). Four weeks after surgery, mice were perfused with silicone and the distribution of intraosseous blood vessels analyzed by μCT. The bone density, μCT microstructure, biomechanical properties, and the immunohistochemical and biochemical indicators of angiogenesis were also measured. The SCI group showed significantly reduced tibial metaphysis bone density, μCT bone microstructure, tibial biomechanical properties, indicators of angiogenesis, and intraosseous blood vessel parameters compared to the NX group. Furthermore, the spinal cord-injured mice exhibited significantly decreased intraosseous blood vessel volume and number during the development of osteoporosis. In conclusion, these data suggest that decreased intraosseous blood vessel volume and number may play an important role in the development of post-nerve-injury osteoporosis. © The Japanese Society for Bone and Mineral Research and Springer 2011.
CITATION STYLE
Ding, W. G., Yan, W. H., Wei, Z. X., & Liu, J. B. (2012). Difference in intraosseous blood vessel volume and number in osteoporotic model mice induced by spinal cord injury and sciatic nerve resection. Journal of Bone and Mineral Metabolism, 30(4), 400–407. https://doi.org/10.1007/s00774-011-0328-y
Mendeley helps you to discover research relevant for your work.