Molecular detection of Zika virus (ZIKV) is a key element of outbreak management. Multiple PCR and isothermal ZIKV assays targeting different ZIKV sequences have been published. In this study, we compared a qRT-PCR, 2 RT-LAMP assays (based on different primer design approaches), and an RT-RPA for the detection of African and Asian/American lineages of ZIKV isolates from human, mosquito, and monkey. Results showed that RT-LAMP detected 100% of samples with a time threshold (Tt) of 18.01 ± 11.71 min while qRT-PCR detected 88.88% of samples with a Tt of 58.30 ± 16.58 min and RT-RPA 50% of samples with a Tt of 3.70 ± 0.44 min.
CITATION STYLE
Diagne, C. T., Faye, M., Lopez-Jimena, B., Abd El Wahed, A., Loucoubar, C., Fall, C., … Sall, A. A. (2020). Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA. In Methods in Molecular Biology (Vol. 2142, pp. 165–179). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0581-3_14
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