Genome engineering using the clustered regularly interspaced short palindromic repeats (CRISPR)- CRISPR associated nuclease 9 (Cas9) technology is revolutionizing biomedical research. CRISPR-Cas9 enables precise editing of genes in a wide variety of cells and organisms, thereby accelerating molecular studies via targeted mutagenesis, epitope tagging, and other custom genetic modifications. Here, we illustrate the CRISPR-Cas9 methodology by focusing on Capicua (Cic), a nuclear transcriptional repressor directly phosphorylated and inactivated by ERK/MAPK. Specifically, we use CRISPR-Cas9 for targeting an ERK docking site of Drosophila Cic, thus generating ERK-insensitive mutants of this important signaling sensor.
CITATION STYLE
Forés, M., Papagianni, A., Rodríguez-Muñoz, L., & Jiménez, G. (2017). Using CRISPR-cas9 to study ERK signaling in Drosophila. In Methods in Molecular Biology (Vol. 1487, pp. 353–365). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6424-6_26
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