Noncoding RNAs, being at the center stage of the organismal development and homeostasis, warrant a detailed analysis to utilize their full therapeutic potential. They form complexes with various proteins that enable the noncoding RNAs to acquire specific cellular functions such as the transcriptional outcomes that are controlled in a spatio-temporal manner. In this protocol, we describe a method to isolate such known (and unknown) protein complexes bound to a nuclear noncoding RNA.
CITATION STYLE
Jayani, R. S., Singh, A., & Notani, D. (2017). Isolation of nuclear RNA-associated protein complexes. In Methods in Molecular Biology (Vol. 1543, pp. 169–185). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6716-2_9
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