A cleanup method for mass spectrometric analysis of sphingosine- and ceramide-1-phosphate in blood and solid tissue using a phosphate capture molecule

Abstract

Cleanup technology and mass spectrometric determination of sphingosine-1-phosphate (S1P) using a phosphate capture molecule are shown. The protocol is rapid, requires neither thin-layer chromatography nor liquid chromatography, and is applicable to both blood and solid tissue samples. The mass spectrometric method is also applicable to ceramide-1-phosphate.