Biological characterization of some virulence factors of Stenotrophomonas maltophilia isolated from urine samples of prostate and bladder cancers patients

Abstract

Stenotrophomonas infections are becoming more widespread around the world. This study included 120 urine samples collected from AL-Amal National Cancer Hospital and Ghazi AL-Hariri Specialized Surgery Hospital / Medical City Hospital, Baghdad/Iraq, cultured on blood agar and MacConkey, then diagnosed by VITEK compact 2 system and studying the virulence factors of Stenotrophomonas maltophilia which included motility, lecitninase production assay, protease activity, lipase activity assay, biofilm formation using tissue culture plate method and siderophore production using M9 medium, and determining the inhibitory effects of Disodium ethylenediaminetetraacetic acid EDTA and curcumin to evaluate the minimum inhibitory concentrations MIC, the results indicated that only 87/120 (72.5%) of urine sample were positive for bacterial growth including different species of bacteria, percentage of bacteria Stenotrophomonas maltophilia was 7/87(8%) from the total bacterial growth, all isolates were showed beta hemolysis activity, motility, and able for producing lecithinase, protease, lipase, the ability to produce biofilm and siderophore, EDTA concentration that inhibit the growth of bacteria was 100, 80 mg/ml which gave inhibition zones of 26mm and 15 mm respectively and curcumin showed antibacterial activity, and the curcumin concentration that inhibit the growth of bacteria was 260 mg/ml which gave an inhibition zone of 14 mm.