Quantitative PCR is the “gold standard” technology to quantify nucleic acids and, since the first report describing real-time PCR detection in 1993, its use has been grown exponentially. More recent technological advancements have extended the field of applications ranging from high-resolution melting detection to digital PCR. Nowadays, it is a very accessible technique, but some pitfalls should be overcome in order to achieve robust and reliable analysis.
CITATION STYLE
Raso, A., & Biassoni, R. (2014). Twenty years of qPCR: A mature technology? Methods in Molecular Biology, 1160. https://doi.org/10.1007/978-1-4939-0733-5_1
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