MCPIP1 regulates fibroblast migration in 3-D collagen matrices downstream of MAP kinases and NF-κB

14Citations
Citations of this article
17Readers
Mendeley users who have this article in their library.

Abstract

The fibroblast-populated three-dimensional (3-D) collagen matrix has been used to model matrix contraction, cell motility, and general fibroblast biology. MCPIP1 (monocyte chemotactic protein-induced protein 1) has been shown to regulate inflammation, angiogenesis, and cellular motility. In the present study, we demonstrated induction of MCPIP1 in human fibroblasts embedded in the stress-released 3-D collagen matrix, which occurred through activation of mitogen-activated protein kinases, phosphoinositide 3-kinase, and NF-κB. Furthermore, MCPIP1 induction was associated with inhibition of fibroblast migration out of the nested collagen matrix. MCPIP1 induction or ectopic expression also upregulated p53. RNA interference of p53 prevented the inhibition of migration produced by induction or ectopic expression of MCPIP1. Our findings suggest a new role for MCPIP1 as a molecular switch that regulates fibroblast migration in the nested collagen matrix model.

Cite

CITATION STYLE

APA

Chao, J., Dai, X., Peña, T., Doyle, D. A., Guenther, T. M., & Carlson, M. A. (2015). MCPIP1 regulates fibroblast migration in 3-D collagen matrices downstream of MAP kinases and NF-κB. Journal of Investigative Dermatology, 135(12), 2944–2954. https://doi.org/10.1038/jid.2015.334

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free