Characterization of the SSAT1 gene and its expression profiling in various tissues and follicles in geese

Abstract

Spermidine/spermine N1-acetyltransferase (SSAT) is a catabolic regulator of polyamines, ubiquitous molecules essential for cell proliferation and differentiation. In this study, the molecular characterization of the SSAT1 gene of the Sichuan white goose was analyzed, as well as its expression profiles in various follicles and tissues. The open reading frame of the SSAT1 cDNA (GenBank No. KM925008) is 516 bp in length and encodes a 171-amino acid protein with a putative molecular weight of 20 kDa. The predicted SSAT1 protein is highly conserved with those of other species, especially Gallus gallus. SSAT1 mRNA was ubiquitously expressed in all the examined tissues. The highest level of SSAT1 mRNA expression was found in the pineal gland (P<0.05), and was 12-fold greater than in the heart. The level of SSAT1 mRNA expression was relatively lower in preovulatory follicles, while it was higher in postovulatory follicles (POFs), particularly in POF1. Furthermore, as postovulatory follicles degenerated, SSAT1 expression gradually decreased. Our findings suggest that SSAT1 might play important roles in mediating the physiological function of the pineal gland and regulating the regression of POFs.