The metaphase chromosome spread technique and subsequent analysis of sister chromatid cohesion is used for (clinical) diagnosis of genetic abnormalities that can cause aberrant sister chromatid cohesion. In addition, the technique can be used to assess the contribution of novel genes to the cohesion establishment and maintenance pathways. Cells are swelled in a hypotonic solution and fixed in Carnoy’s solution. Samples are then dropped onto glass slides, and the spread chromosomes are stained and visualized by microscopy. Defects in sister chromatid cohesion can be easily assessed using this method, examples of which are given.
CITATION STYLE
Feeney, K. M., McFarlane-Majeed, L., & Parish, J. L. (2014). Analyzing sister chromatid cohesion in mammalian cells. Methods in Molecular Biology, 1170, 563–569. https://doi.org/10.1007/978-1-4939-0888-2_32
Mendeley helps you to discover research relevant for your work.