Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affinity binders from libraries of folded proteins. It has been successfully applied to single-chain Fv fragments of antibodies and alternative scaffolds, such as Designed Ankyrin Repeat Proteins (DARPins). High-affinity binders with new target specificity can be obtained from highly diverse DARPin libraries in only a few selection rounds. In this protocol, the selection from the library and the process of affinity maturation and off-rate selection are explained in detail. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Dreier, B., & Plückthun, A. (2012). Rapid selection of high-affinity binders using ribosome display. Methods in Molecular Biology, 805, 261–286. https://doi.org/10.1007/978-1-61779-379-0_15
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