2 Amino 2 methyl 1 propanol as the alkalizing agent in an improved continuous flow cresolphthalein complexone procedure for calcium in serum

Abstract

An improved cresolphthalein complexone procedure is described for calcium in which diethylamine is replaced by a nontoxic amino alcohol. Advantages of the procedure are increased sensitivity, excellent baseline stability, a more optimum reaction environment (pH 10.0) that eliminates blanking problems, and freedom from interference by magnesium. Values obtained by this method are not significantly different (P<0.05) from those obtained by atomic absorption spectrophotometry.