Alkaline Protease Production Using Proteinaceous Tannery Solid Waste

Abstract

Leather industry has commonly been associated with high pollution due to the bad smell, organic wastes and high water consumption caused during traditional manufacturing processes. Different forms of waste in quality and quantity, which emerge during the transformation of hides and skins into leathers in thousands of leather factories, from primitive to modern all around the world, have negative impacts on the environment. In leather factories solid waste containing protein that constitute more than 60% of raw hide weight are disposed to the environment without turning them into good use at industrial level. A biological approach such as alkaline protease production by different microorganism is prominent way to utilization of tannery proteinaceous solid waste. In this research work four different species Bacillus licheniformis, Bacillus coagulans, Bacillus polymxa and Bacillus amyloliquefaciens were isolated from soil sample and identified by biochemical tests. These species efficiently produce alkaline using animal fleshing (ANFL) which is an untanned tannery solid waste as prominent source of protein .The efficiency of protease production was measured on different temperature, pressure and incubation time. Bacillus licheniformis gave highest protease production. Due to efficient production of alkaline protease by same bacillus species, it can be use at industrial level.