Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2

Abstract

Two-dimensional MXene nanosheets with vertical junction structure was employed for easy immobilization of horse radish peroxidase enzymes to fabricate the electrochemical hydrogen peroxide (H2O2) biosensor. The synthesized MXene nanosheets exhibited large specific area, excellent electronic conductivity and good dispersion in aqueous phase. Horse Radish Peroxidase (HRP) enzymes molecules immobilized on MXene/chitosan/GCE electrode demonstrated good electrocatalytic activity toward reduction of H2O2. The fabricated HRP@MXene/chitosan/GCE biosensor exhibited a wide linear range from 5 to 1650 μmol∙L-1, a limit of detection of 0.74 μmol∙L-1 and good operation stability. The fabricated biosensor was successfully employed for detection of trace level of H2O2 in both solid and liquid food.