AlphaScreen ® SureFire ® assay is a novel technology that combines luminescent oxygen channeling technology, nano-beads, and monocloncal antibodies to detect the level of a selected protein in a volume lower than 5 μl. This method is more sensitive compared with the traditional enzyme-linked immunosorbent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen ® SureFire ® assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by L-α-lysophosphatidylinositol (LPI) and certain cannabinoids.
CITATION STYLE
Anavi-Goffer, S., & Ross, R. A. (2016). A functional assay for GPR55: Envision protocol. In Methods in Molecular Biology (Vol. 1412, pp. 77–83). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3539-0_8
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