Carbohydrate carriers affect adhesion of H. pylori to immobilized Leb-oligosaccharide

Abstract

The present study involved comparison of adhesion of Helicobacter pylori KH202 to immobilized Leb-oligosaccharide carried on different carriers, i.e. Leb-oligosaccharide conjugated with polyacrylamide, bovine serum albumin, and dipalmitoylphosphatidylethanolamine (Leb-PAA, Leb-BSA, and Leb-DPPE). All of the Leb-oligosaccharide-carrying neoglycoconjugates served as ligands for H. pylori. However, H. pylori required 10-fold and 100-fold quantities of Leb-antigen to adhere to Leb-PAA and to Leb-DPPE in comparison to the quantity of Leb-antigen needed to adhere to Leb-BSA, respectively. H. pylori adhesion to Leb-PAA and Leb-DPPE was clearly inhibited by Leb-oligosaccharide, but adhesion to Leb-BSA was hardly inhibited by the oligosaccharide. Therefore, the carbohydrate carrier affects the affinity of H. pylori KH202 toward Leb-antigen, although the bacteria recognize Leb-antigen regardless of the carbohydrate carrier. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.