Stable isotope labeling by amino acids in cell culture (SILAC) and iodoacetyl tandem mass tag (iodoTMT) are well-implemented mass spectrometry-based approaches for quantification of proteins and for site-mapping of cysteine modification. We describe here a combination of SILAC and iodoTMT to assess ongoing changes in the global proteome and cysteine modification levels using liquid chromatography separation coupled with high-resolution mass spectrometry (LC-MS/MS).
CITATION STYLE
Vajrychova, M., Salovska, B., Pimkova, K., Fabrik, I., & Hodny, Z. (2023). SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status. In Methods in Molecular Biology (Vol. 2603, pp. 259–268). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2863-8_21
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