Three-dimensional culture systems reflect biological environments better than conventional two-dimensional culture. Additionally, three-dimensional culture is a strong experimental tool to analyze direct interactions between cancer cells and stromal cells in vitro. Herein, we describe protocols for an organotypic fat invasion model that is a novel culturing system mimicking the extrapancreatic invasion of pancreatic adenocarcinoma (PDAC). This novel model is based on the collagen I gel embedding method and enables us to analyze the functional and histological interactions between cancer cells and adipose tissue.
CITATION STYLE
Okumura, T., Ohuchida, K., & Nakamura, M. (2019). An in vitro three-dimensional organotypic model to analyze peripancreatic fat invasion in pancreatic cancer: A culture system based on collagen gel embedding. In Methods in Molecular Biology (Vol. 1882, pp. 135–141). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8879-2_11
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