Gametocidal (Gc) system is a novel method of inducing chromosome mutations in wheat by using Gc genes of wheat-related species. Many deletion lines were effi ciently produced by this system and used for physical mapping of genes and DNA markers. A large stocks with chromosome deletions in homozygous state as well as the lines with Gc genes are available in the gene bank of Japan (KOMUGI). In this chapter, I describe the method of inducing breakage in a target chromosome using lines with the Gc gene on chromosome 2C of Aegilops cylindrica (a wheat-related wild species) and nullisomic-tetrasomics.
CITATION STYLE
Tsujimoto, H. (2016). Gametocidal system for dissecting wheat chromosomes. In Methods in Molecular Biology (Vol. 1469, pp. 101–109). Humana Press Inc. https://doi.org/10.1007/978-1-4939-4931-1_7
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