Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology

Abstract

PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem. Copyright © 2008, American Society for Microbiology. All Rights Reserved.