Selected/multiple reaction monitoring-mass spectrometry (SRM/MRM) is an analytical method that is frequently combined to the use of stable isotope-labeled standard (SIS) peptides for absolute protein quantification. The application of SRM/MRM is a relatively recent development in the proteomics field for analysis of biological samples (plasma, urine, cell/tissue lysates) targeting to a large extent biomarker validation. Although MRM generally by-passes the use of antibodies (being linked to sub-optimal assay specificity in many cases), bioanalytical validation of MRM protocols has not been robustly applied because of sensitivity issues, in contrary to antibody-based methods. In this chapter, we will discuss the points that should be addressed for MRM method development in clinical proteomics applications.
CITATION STYLE
Kontostathi, G., Makridakis, M., Bitsika, V., Tsolakos, N., Vlahou, A., & Zoidakis, J. (2019). Development and validation of multiple reaction monitoring (MRM) assays for clinical applications. In Methods in Molecular Biology (Vol. 1959, pp. 205–223). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9164-8_14
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