Coulometric electrochemical detection of phospholipid hydroperoxides by high-performance liquid chromatography

Abstract

A technique for the sensitive and convenient determination of phosphatidylcholine hydroperoxides was developed that uses coulometric electrochemical detection in a high-performance liquid chromatographic analysis. The detection limit for phosphatidylcholine hydroperoxides by this method was 20 pmol at a signal-to-noise ratio of 3. The glutathione-dependent phospholipid hydroperoxide-reducing activity of fresh human plasma was ascertained by this method. © 1997, Taylor & Francis Group, LLC. All rights reserved.