ISLIM: A comprehensive approach to mapping and characterizing gene regulatory networks

Abstract

Mapping gene regulatory networks is a significant challenge in systems biology, yet only a few methods are currently capable of systems-level identification of transcription factors (TFs) that bind a specific regulatory element. We developed a microfluidic method for integrated systems-level interaction mapping of TF-DNA interactions, generating and interrogating an array of 423 fulllength Drosophila TFs. With integrated systemslevel interaction mapping, it is now possible to rapidly and quantitatively map gene regulatory networks of higher eukaryotes © 2012. Published by Oxford University Press.