Structure-based site-directed affinity maturation of antibodies can be expanded by multiple-point mutations to obtain various mutants. However, selecting the appropriate number of promising mutants for experimental evaluation from the vast number of combinations of multiple-point mutations is challenging. In this report, we describe how to narrow candidate mutants using the so-called weak interaction analysis such as CH–π and CH–O in addition to widely recognized interactions such as hydrogen bonds.
CITATION STYLE
Chiba, S., Okuno, Y., & Ohta, M. (2023). Structure-Based Affinity Maturation of Antibody Based on Double-Point Mutations. In Methods in Molecular Biology (Vol. 2552, pp. 323–331). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2609-2_18
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