Stable isotope labelling by amino acids in cell culture (SILAC) is a technique that allows proteomic profiling of cells. In this chapter we describe a protocol for the identification and quantification of newly synthesised proteins. The methodology can be applied to any cultured cell system with relevance to schizophrenia, affective disorders and autism spectrum conditions including those addressing responses to pharmacological stimuli.
CITATION STYLE
Duque-Guimarães, D., Ong, T. P., de Almeida-Faria, J., Guest, P. C., & Ozanne, S. E. (2017). SILAC mass spectrometry profiling: A psychiatric disorder perspective. In Advances in Experimental Medicine and Biology (Vol. 974, pp. 289–298). Springer New York LLC. https://doi.org/10.1007/978-3-319-52479-5_27
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