The protein-lipid overlay assay is an inexpensive, easy-to-implement, and high-throughput methodology that employs nitrocellulose membranes to immobilize lipids in order to rapid screen and identify protein-lipid interactions. In this chapter, we show how this methodology can identify potential modulators of protein-lipid interactions by screening water-soluble lipid competitors or even the introduction of pH changes during the binding assay to identify pH-dependent lipid binding events.
CITATION STYLE
Tang, T. X., Xiong, W., Finkielstein, C. V., & Capelluto, D. G. S. (2017). Identification of lipid binding modulators using the protein-lipid overlay assay. In Methods in Molecular Biology (Vol. 1647, pp. 197–206). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7201-2_13
Mendeley helps you to discover research relevant for your work.