Detection of the intercellular adhesion loci (ica) in clinical Staphylococcus aureus strains responsible for hospital acquired auricular infection

Abstract

In clinical Staphylococcus aureus strains, the presence of the ica genes, biofilm formation and susceptibility to antibiotics are considered important factors of virulence. In this study, 35 strains of S. aureus, isolated from auricular infection, were investigated for slime production using Congo red agar (CRA) method, antibiotic susceptibility, presence of mecA gene, and presence of icaA and icaD gene. The results show that 60% of strains were mecA positive when tested by PCR although 25.7% of strains were oxacillin resistant when tested with ATB STAPH. Qualitative slime production of S. aureus using CRA revealed that 74.3% of S. aureus were slime producers. All the strains carried the ica gene. Copyright © Source Interlink Media.