Histo-blood group antigen (HBGA) recognition by norovirus (NoV) has been studied using various techniques. Enzyme-linked immunosorbent assays (ELISAs) using virus-like particles (VLPs) have enabled us to visualize the last step of HBGAs-NoV binding with a total reaction time of approximately 8 h. Herein, we describe two ELISA-based methods to detect and quantify NoV VLP attachment to HBGAs: saliva-VLP binding assay and carbohydrate-VLP binding assay.
CITATION STYLE
Shirato, H. (2020). ELISA-Based methods to detect and quantify norovirus virus-like particle attachment to histo-blood group antigens. In Methods in Molecular Biology (Vol. 2132, pp. 597–607). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0430-4_51
Mendeley helps you to discover research relevant for your work.