Electrophysiological measurement of the number of rhodopsin molecules in single Limulus photoreceptors

Abstract

Two partly independent electrophysiological methods are described for measuring the number of rhodopsin molecules (R) in single ventral photoreceptors. Method 1 is based on measurements of the relative intensity required to elicit a quantal response and the relative intensity required to half-saturate the early receptor potential (ERP). Method 2 is based on measurements of the absolute intensity required to elicit a quantal response. Both methods give values of R ≈ 109. From these and other measurements, estimates are derived for the surface density of rhodopsin (8,000/µ.m2), the charge movement during the ERP per isomerized rhodopsin (20 × 10-21 C), and the half-time for thermal isomerization of rhodopsin (36 yr). © 1977, Rockefeller University Press., All rights reserved.