RNA elements in the untranslated regions of plus-strand RNA viruses can control a variety of viral processes including translation, replication, packaging, and subgenomic mRNA production. The 30 untranslated region (30UTR) of Tobacco necrosis virus strain D (TNV-D; genus Betanecrovirus, family Tombusviridae) contains several well studied regulatory RNA elements. Here, we explore a previously unexamined region of the viral 30UTR, the sequence located upstream of the 30-cap independent translation enhancer (30CITE). Our results indicate that (i) a long-range RNA-RNA interaction between an internal RNA element and the 30UTR facilitates translational readthrough, and may also promote viral RNA synthesis; (ii) a conserved RNA hairpin, SLX, is required for efficient genome accumulation; and (iii) an adenine-rich region upstream of the 30CITE is dispensable, but can modulate genome accumulation. These findings identified novel regulatory RNA elements in the 30UTR of the TNV-D genome that are important for virus survival.
CITATION STYLE
Newburn, L. R., Wu, B., & Andrew White, K. (2020). Investigation of novel RNA elements in the 30UTR of tobacco necrosis virus-D. Viruses, 12(8). https://doi.org/10.3390/v12080856
Mendeley helps you to discover research relevant for your work.