This study examined the chemical structural characteristics of cellulolytic enzyme lignin isolated from switchgrass focusing on comparisons between wild-type control and caffeic acid 3-O-methyltransferase (COMT) down-regulated transgenic line. Nuclear magnetic resonance techniques including 13 C, 31 P, and two-dimensional 13 C- 1 H heteronuclear single quantum coherence as well as gel permeation chromatography were employed. Compared to the wild-type, the COMT down-regulated transgenic switchgrass lignin demonstrated a decrease in syringyl (S):guaiacyl (G) ratio and p-coumarate:ferulate ratio, an increase in relative abundance of phenylcoumaran unit, and a comparable content of total free phenolic OH groups along with formation of benzodioxane unit. In addition, COMT down-regulation had no significant effects on the lignin molecular weights during its biosynthesis process.
CITATION STYLE
Samuel, R., Pu, Y., Jiang, N., Fu, C., Wang, Z. Y., & Ragauskas, A. (2014). Structural characterization of lignin in wild-type versus COMT down-regulated switchgrass. Frontiers in Energy Research, 1(JAN). https://doi.org/10.3389/fenrg.2013.00014
Mendeley helps you to discover research relevant for your work.