Electrophoretic separation and detection of metalloproteins by x-ray fluorescence mapping

Abstract

All living systems depend on metalloproteins. Yet, while tools for the separation and identification of apo-proteins are well developed, those enabling identification and quantitation of individual metalloproteins within complex mixtures are still nascent. Here, we describe the electrophoretic separation of a mixture of carbonic anhydrase, ceruloplasmin, urease, and hemoglobin using native 2D gel electrophoresis and X-ray fluorescence mapping - an approach we have developed to be broadly applicable, not require specialized equipment for sample preparation, and likely to be extensible in the future. © 2012 Springer Science+Business Media, LLC.