A rapid and simple enzyme immunoassay system was developed for detection of the food-borne pathogen Escherichia coli O157. This system is based on the use of anti-E. coli O157 antibody-coated Polymacron(TM), an inexpensive macroporous polyester fabric, as a high-surface-area immunoadsorbent for the rapid capture and subsequent immunoenzymatic detection of E. coli O157 antigens extracted from test samples by heating at 100°C for 10 min in the presence of sodium cholate. A dot blot format was used which facilitated the assay of multiple samples. The method was specific for all strains tested bearing the O157 and related antigens (e.g., group N Salmonella), giving positive reactions in the assay of pure cultures of 29 E. coli O157:H7 strains, one E. coli O157:H12, one E. coli O157:NM (nonmotile) and one Salmonella urbana strain, but not in the assay of a variety of other gram-negative and gram-positive bacteria. The method permitted the detection of ca. 400 E. coli O157:H7 CFU in a 5-μl spot applied to Polymacron(TM), and of as few as 0.4 CFU of E. coli O157:H7 cells per g inoculated into ground beef samples, which were assayed after overnight enrichment.
CITATION STYLE
Blais, B. W., Booth, R. A., Phillippe, L., Pandian, S., & Yamazaki, H. (1997). Polymacron(TM) enzyme immunoassay system for detection of Escherichia coli O157 inoculated into foods. Journal of Food Protection, 60(2), 98–101. https://doi.org/10.4315/0362-028X-60.2.98
Mendeley helps you to discover research relevant for your work.